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Description Doxycycline Rapid Test Kit   Principle Doxycycline rapid test kit is based on lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Doxycycline in the sample, a visible T band will appear; T band is invisible if it has more Doxycycline in the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Doxycycline  in the tissue, honey, egg, milk , fish sample.   Technical Specification                                                 Detect limit： 200ppb            Reaction temperature: 25℃ Detect time:   30min.   Materials provided                                                  Rapid Test Card 20PCS PBS solution     50mL   Materials required not provided                                         Deionized water, Ethyl acetate Centrifuge tube:  5mL, 10mL Micropipettors:  20~200 µL, 100~ 1000 µL Vortex, centrifuge, balance (0.01 g), homogenizer, shaker   Sample Preparation                                              Take 5±05g of the homogenized sample, add 5mL deionized water, then add 5mL ethyl acetate , shake for 5min properly. Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 10 min. Transfer 3mL supernatant, evaporate to dryness by nitrogen at 50~60 ℃. Dissolve the dry residue in 200uL PBS solution , mix for 30s. Take 80µL for analysis.   Sample Determination                                                                                      Take out the cassette from the pack and place it horizontally. Return to room temperature (20-25℃), determine the cassette immediately. Add 2 drops(Approx 80µL) sample extraction, react for 5-10 min and read result. Result Judgement Positive: The presence of “C” band, no matter “T” It means that Doxycycline in the sample extraction is higher than 200ppb. Negative: Both clear“C” band and “T” band appears. It means that Doxycycline in the sample extractionis lower than 200ppb . Invalid: No colored band appears in C zone, no matter whether T band appears.