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EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. This fast, one-step mix is designed to work with a variety of instruments? default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms.Features of the EXPRESS One-Step Superscript qRT-PCR Kit include: ? Fast-activating, antibody-mediated Platinum Taq DNA Polymerase?complete, rapid activation; provides high sensitivity and reproducibility ? The One-Step SuperScript III Reverse Transcriptase module? a unique formulation to ensure high yield and less PCR inhibition ? Flexible formats with separate or pre-mixed ROX?designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler? 480, Corbett Rotor-Gene?) ? Contains heat-labile UDG?significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40?C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50?C, at which temperature the heat-labile UDG is totally and irreversibly inactivatedProduct More SpecificationsSpecification

No. of Reactions
500 Reactions
Polymerase
Taq DNA Polymerase
Sample Type
RNA
For Use With (Equipment)
7500 Fast System, 7500 System,Applied Biosystems StepOnePlus? Fast Real-Time PCR System, BioRad CFX96?, StepOnePlus?, Fast Mode, StepOnePlus?, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
PCR Method
1-Step qRT-PCR
Sufficient For
500 Reactions
Product Type Specs
qRT-PCR Kit
Format
Tube
Product Line
SuperScript?
Detection Method
Primer-Probe Detection
Reaction Speed
Fast, Standard
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