DNA sequencing is a laboratory technique used to determine the order of nucleotides (A, T, C, G) in a DNA molecule. It is a crucial tool for many areas of research, including genetics, genomics, and molecular biology.
There are different methods for DNA sequencing, but the most commonly used method is called Sanger sequencing, which was developed by Frederick Sanger in 1977. Here are the basic steps involved in Sanger sequencing:
DNA Fragmentation: The DNA molecule is first fragmented into smaller pieces of a few hundred bases in length.
Amplification: Each fragment is then amplified using a technique called PCR (Polymerase Chain Reaction) to produce multiple copies of the fragment.
Labeling: During amplification, a small amount of modified DNA bases (ddNTPs) are added to the reaction. These modified bases terminate DNA strand elongation when incorporated into the growing DNA strand.
Separation: The labeled fragments are then separated based on their size using gel electrophoresis. The fragments are loaded onto a gel matrix, and an electric current is applied to separate the fragments by size. The smallest fragments move the farthest, and the largest fragments remain closest to the origin.
Detection: After separation, the labeled fragments can be detected using fluorescent tags or radioactivity.
Analysis: Finally, the sequence of the DNA molecule can be determined by analyzing the labeled fragments based on their size and position on the gel.
Sanger sequencing is a reliable and accurate method for DNA sequencing, but it is also time-consuming and labor-intensive, and can only sequence a limited length of DNA at a time. In recent years, newer sequencing technologies, such as Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS), have emerged, which offer faster and more comprehensive sequencing of DNA.
DNA Structure Model - 5 Parts Mounted on Base
Set of 5 different models detailing DNA structures are mounted on a common wooden base for classroom observation
Large model with DNA structures magnified 1000's of times provides comprehensive detail of molecule structure, mechanisms of replication, and nucleotide base pairing
Expertly designed by biologists and educators for the highest degree of detail and accuracy
Custom molded from PVC plastic for durability and hand-painted by skilled artists to deliver the highest level of detail
Structures are clearly labeled for easy identification
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Description
Savant SpeedVac DNA 130 Integrated Vacuum Concentrator System
Thermo Scientific Savant SpeedVac DNA130 is a compact, integrated vacuum concentrator designed for fast, efficient concentration of DNA and RNA applications. SpeedVac™ DNA130 vacuum concentrator will support sample preparations with ease and efficiency.
Compact, easy to use design: smallest integrated benchtop unit saves lab bench space.
Corrosion-resistant, PTFE coated chamber offers years of service
Built-in, oil free vacuum pump offers maintenance-free operation
Includes a rotor that holds 36 x 1.5-2.0 ml tubes (RD36)
Operating Features:
Consistent performance: designed to ensure consistent drying times and reproducible results
User-friendly interface
Three user defined programs that can be modified and saved
Temperature set point: ambient, 35°C to 65°C in 5°C increments
Heat Time setting: from 1 m to 9h 59 m or continuous
Run Time setting: from 1m to 9h 59m
Audible alarm to alert user when run is complete
Sample Protection
Dry at ambient temperatures or using low heat to avoid sample denaturing
Oil-free diaphragm pump provides gentle transition from atmosphere to ultimate vacuum setting with no solvent bumping
Safety Features
PTFE-coated chamber resists chemicals and cleans easily
Latched lid and lid lock indicator ensures user safety during operation
Lid stay mechanism to secure lid while it‘s open, allowing user to have both hands free for operation
Unit and pump are virtually maintenance-free
External post trap to neutralize ammonium hydroxide vapors
Optional chemical traps and optional cartridges to trap radioactive chemicals to minimize concerns in handling hazardous fumes and chemicals.
Solvents and Solvent Combinations:
Water
PCR buffers (aqueous)
Ethanol and water
Ammonium Hydroxide (will require an ammonia post-trap ANT-100 and neutralizing solution accessory ANS121)
Product Overview?DNAPac? PA200 Oligonucleotide HPLC Columns
Catalog number: 063000
Related applications: Industrial Chromatography
Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific? DNAPac? PA200 Oligonucleotide HPLC Columns.Product More Specificationsspecification_codeColumn FormatAnalytical ColumnDiameter (Metric)4 mmFlow Rate1.2 mL/minFor Use WithUltiMate 3000 Titanium SystemLength (Metric)250 mmMax. Pressure4000 psi (275 bar)Mobile Phase CompatibilitypH 2 to 12.5, 100% compatible with common organic solvents, Ionic form eluents: chloride, perchlorateParticle Size8 ?mpH2.5 to 12.5Pore SizeNonporousTemperature85?CStationary PhaseQuaternary AmmoniumProduct LineDNAPacPacking MaterialSpherical, Nonporous PolymerColumn TypeIon ExchangePhaseIon ExchangeTypeAnalytical ColumnTechniqueNucleic Acid/Anion ExchangeUnit SizeEachMade InUSAWrite a review
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Applications:
? Removes DNA from protein preparations and RNA samples
? Mediates nick translation
? Generates random fragments for dideoxy sequencing
? Degrade template DNA following in vitro transcription
? Mediate DNase I foot-printingWrite a review
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Product Overview82359
DNase Test Agar BaseConcentration 42.00 gm/lit.
Part D
Storage : 8 to 25?C (Cool & dry area)
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review
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Product OverviewDNase Test Agar Base82359Concentration 42.00 gm/lit.
Part D
Storage: 8 to 25?C (Cool & dry area)
HSN Code: 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)Package Size: 100 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review
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Product Overview?M482
DNase Test Agar Base
for detection of deoxyribounclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Staphylococcus aureus on M482Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review
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DNAZap? Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. Features of DNAZap? Solutions:
? Completely degrades contaminating DNA and RNA at the level of PCR sensitivity
? Works on contact
? Degrades nucleic acid, unlike other products on the market that only act as detergents
? Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tubes
Using DNAZap? Solutions
PCR and related amplification techniques produce large amounts of a target nucleic acid sequence. This exquisite sensitivity creates its own drawbacks, primarily the potential amplification of undesirable, contaminating nucleic acids along with the desired sequence?and sometimes at the expense of the desired sequence. DNAZap??Solutions provide a convenient method to decontaminate a variety of surfaces in a matter of minutes. It even effectively eliminates contaminating DNA in PCR tubes without inhibiting subsequent enzymatic reactions. All contaminating nucleic acid is degraded to nucleotides, preventing any chance of false positive amplification.Product More SpecificationsMade InUSAThere are no reviews for this product.Write a review
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Product OverviewDNAzol? BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.? Specifically formulated for extraction of genomic DNA from whole blood
? Optimized for the rapid Isolation of genomic DNAEfficient Isolation of Genomic DNA from Whole Blood
DNAzol? BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol? BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol? Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 ?g DNA. In addition to the isolation of genomic DNA, DNAzol? BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.Rapid Isolation of Genomic DNA
The DNAzol? Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol? Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol? Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.Product More Specificationsspecification_codeHigh-throughput CompatibilityNot High-throughput Compatible (Manual)Shipping ConditionRoom TemperatureProduct LineDNAzol?For Use With (Application)PCR, Southern Blotting, Sequencing, CloningFinal Product TypeGenomic DNAPurification TargetGenomic DNASample TypeBloodPurification Time30 minIsolation TechnologyOrganic ExtractionMade InUSAWrite a review
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Product OverviewDNeasy Blood & Tissue Kits
For spin-column or 96-well extraction of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses
Features
Standardized method for a variety of sample types
High yields even from specialized samples
High-quality DNA
Optimized protocols for a range of starting materials
Spin-column and 96-well high-throughput formats
DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA extraction without phenol or chloroform in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible extraction of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Extraction of DNA using the DNeasy Blood & Tissue Kit.DNeasy Blood & Tissue standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the DNeasy Blood & Tissue protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.Product More SpecificationsMade InGermanyWrite a review
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Product OverviewDNeasy Plant Pro and Plant Kits
For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds
Features
Superior PCR performance with patented Inhibitor Removal Technology (IRT)
Rapid extraction of ready-to-use DNA
No organic extraction, no ethanol precipitation
Highly efficient lysis and release of DNA from tough plant materials and associated plant pathogens
The DNeasy Plant Pro Kit has several innovative features to overcome the challenges of DNA extraction from plant tissue and enable recovery of high-quality DNA from the toughest sample types, including strawberry leaf, grapevine leaf, pine needles and various seed types. Increased yields of plant DNA and plant pathogen DNA combined with superior removal of inhibitors ensure high-performance results in sensitive downstream applications.DNeasy Plant Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3?260 ?g of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the DNeasy silica membrane. DNeasy Plant Kits also provide silica-based DNA purification in a convenient 96-well plate format with typical yields of 1?15 ?g DNA, depending on the plant species.Package Size: 50 KitProduct More SpecificationsMade InUSAThere are no reviews for this product.Write a review
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ZirGo Alfasigma 12 Sachets ₦43,491.00
Stimmun 7 Lindas Gtt 50ml ₦30,685.00
Cyclocel 500Mg Injection 1?s ₦27,200.00
Stardea Kelofer C 14 Sachets ₦26,847.00
DNA sequencing is a laboratory technique used to determine the order of nucleotides (A, T, C, G) in a DNA molecule. It is a crucial tool for many areas of research, including genetics, genomics, and molecular biology. There are different methods for DNA sequencing, but the most commonly used method is called Sanger sequencing, which was developed by Frederick Sanger in 1977. Here are the basic steps involved in Sanger sequencing:
DNA Structure Model - 5 Parts Mounted on Base Set of 5 different models detailing DNA structures are mounted on a common wooden base for classroom observation Large model with DNA structures magnified 1000's of times provides comprehensive detail of molecule structure, mechanisms of replication, and nucleotide base pairing Expertly designed by biologists and educators for the highest degree of detail and accuracy Custom molded from PVC plastic for durability and hand-painted by skilled artists to deliver the highest level of detail Structures are clearly labeled for easy identification
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Description Savant SpeedVac DNA 130 Integrated Vacuum Concentrator System Thermo Scientific Savant SpeedVac DNA130 is a compact, integrated vacuum concentrator designed for fast, efficient concentration of DNA and RNA applications. SpeedVac™ DNA130 vacuum concentrator will support sample preparations with ease and efficiency. Compact, easy to use design: smallest integrated benchtop unit saves lab bench space. Corrosion-resistant, PTFE coated chamber offers years of service Built-in, oil free vacuum pump offers maintenance-free operation Includes a rotor that holds 36 x 1.5-2.0 ml tubes (RD36) Operating Features: Consistent performance: designed to ensure consistent drying times and reproducible results User-friendly interface Three user defined programs that can be modified and saved Temperature set point: ambient, 35°C to 65°C in 5°C increments Heat Time setting: from 1 m to 9h 59 m or continuous Run Time setting: from 1m to 9h 59m Audible alarm to alert user when run is complete Sample Protection Dry at ambient temperatures or using low heat to avoid sample denaturing Oil-free diaphragm pump provides gentle transition from atmosphere to ultimate vacuum setting with no solvent bumping Safety Features PTFE-coated chamber resists chemicals and cleans easily Latched lid and lid lock indicator ensures user safety during operation Lid stay mechanism to secure lid while it‘s open, allowing user to have both hands free for operation Unit and pump are virtually maintenance-free External post trap to neutralize ammonium hydroxide vapors Optional chemical traps and optional cartridges to trap radioactive chemicals to minimize concerns in handling hazardous fumes and chemicals. Solvents and Solvent Combinations: Water PCR buffers (aqueous) Ethanol and water Ammonium Hydroxide (will require an ammonia post-trap ANT-100 and neutralizing solution accessory ANS121)
Product Overview?DNAPac? PA200 Oligonucleotide HPLC Columns Catalog number: 063000 Related applications: Industrial Chromatography Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific? DNAPac? PA200 Oligonucleotide HPLC Columns.Product More Specificationsspecification_codeColumn FormatAnalytical ColumnDiameter (Metric)4 mmFlow Rate1.2 mL/minFor Use WithUltiMate 3000 Titanium SystemLength (Metric)250 mmMax. Pressure4000 psi (275 bar)Mobile Phase CompatibilitypH 2 to 12.5, 100% compatible with common organic solvents, Ionic form eluents: chloride, perchlorateParticle Size8 ?mpH2.5 to 12.5Pore SizeNonporousTemperature85?CStationary PhaseQuaternary AmmoniumProduct LineDNAPacPacking MaterialSpherical, Nonporous PolymerColumn TypeIon ExchangePhaseIon ExchangeTypeAnalytical ColumnTechniqueNucleic Acid/Anion ExchangeUnit SizeEachMade InUSAWrite a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Applications: ? Removes DNA from protein preparations and RNA samples ? Mediates nick translation ? Generates random fragments for dideoxy sequencing ? Degrade template DNA following in vitro transcription ? Mediate DNase I foot-printingWrite a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product Overview82359 DNase Test Agar BaseConcentration 42.00 gm/lit. Part D Storage : 8 to 25?C (Cool & dry area) HSN Code : 38210000 IMDG Identification :Not Regulated for Transport (Non-Haz)Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product Overview?M482 DNase Test Agar Base for detection of deoxyribounclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Staphylococcus aureus on M482Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
DNAZap? Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. Features of DNAZap? Solutions: ? Completely degrades contaminating DNA and RNA at the level of PCR sensitivity ? Works on contact ? Degrades nucleic acid, unlike other products on the market that only act as detergents ? Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tubes Using DNAZap? Solutions PCR and related amplification techniques produce large amounts of a target nucleic acid sequence. This exquisite sensitivity creates its own drawbacks, primarily the potential amplification of undesirable, contaminating nucleic acids along with the desired sequence?and sometimes at the expense of the desired sequence. DNAZap??Solutions provide a convenient method to decontaminate a variety of surfaces in a matter of minutes. It even effectively eliminates contaminating DNA in PCR tubes without inhibiting subsequent enzymatic reactions. All contaminating nucleic acid is degraded to nucleotides, preventing any chance of false positive amplification.Product More SpecificationsMade InUSAThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product OverviewDNAzol? BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.? Specifically formulated for extraction of genomic DNA from whole blood ? Optimized for the rapid Isolation of genomic DNAEfficient Isolation of Genomic DNA from Whole Blood DNAzol? BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol? BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol? Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 ?g DNA. In addition to the isolation of genomic DNA, DNAzol? BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.Rapid Isolation of Genomic DNA The DNAzol? Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol? Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol? Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.Product More Specificationsspecification_codeHigh-throughput CompatibilityNot High-throughput Compatible (Manual)Shipping ConditionRoom TemperatureProduct LineDNAzol?For Use With (Application)PCR, Southern Blotting, Sequencing, CloningFinal Product TypeGenomic DNAPurification TargetGenomic DNASample TypeBloodPurification Time30 minIsolation TechnologyOrganic ExtractionMade InUSAWrite a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product OverviewDNeasy Blood & Tissue Kits For spin-column or 96-well extraction of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses Features Standardized method for a variety of sample types High yields even from specialized samples High-quality DNA Optimized protocols for a range of starting materials Spin-column and 96-well high-throughput formats DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA extraction without phenol or chloroform in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible extraction of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Extraction of DNA using the DNeasy Blood & Tissue Kit.DNeasy Blood & Tissue standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the DNeasy Blood & Tissue protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.Product More SpecificationsMade InGermanyWrite a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product OverviewDNeasy Plant Pro and Plant Kits For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds Features Superior PCR performance with patented Inhibitor Removal Technology (IRT) Rapid extraction of ready-to-use DNA No organic extraction, no ethanol precipitation Highly efficient lysis and release of DNA from tough plant materials and associated plant pathogens The DNeasy Plant Pro Kit has several innovative features to overcome the challenges of DNA extraction from plant tissue and enable recovery of high-quality DNA from the toughest sample types, including strawberry leaf, grapevine leaf, pine needles and various seed types. Increased yields of plant DNA and plant pathogen DNA combined with superior removal of inhibitors ensure high-performance results in sensitive downstream applications.DNeasy Plant Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3?260 ?g of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the DNeasy silica membrane. DNeasy Plant Kits also provide silica-based DNA purification in a convenient 96-well plate format with typical yields of 1?15 ?g DNA, depending on the plant species.Package Size: 50 KitProduct More SpecificationsMade InUSAThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue