Description

PRODUCT DESCRIPTION

Bead Mill Fecal DNA Purification Mini Kit – 50 Prep
The Omni Fecal DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from stool and urine. After sample lysis using the Omni pre-filled bead beating tubes, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Fecal DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.
Product Features:

Complete DNA purification kit including spin-columns and nontoxic reagents
Effective removal of PCR inhibitors including humic compounds
Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
Isolation of pure DNA from up to 250 mg stool, biosolids, gut material and urine
Rapid sample disruption with 0.5 mm glass beads
Recover up to 25 ug DNA per filter

Illustrated Protocol
 
Ordering Information: Unit includes 50 spin-columns, 50 pre-filled bead tubes with 0.5 mm glass beads, reagents suitable for 50 purifications and instruction guide

Specifications

DNA types:Microbial genomic DNA
DNA purity:High quality genomic DNA with A260/A280 > 1.8 
Inhibitor removal:DNA is separated from humic compounds and other PCR inhibitors prior to purification
DNA yield:Up to 25 ug per filter 
Capacity:Capable of microbial DNA extraction from up to 250 mg of stool, gut material, water and urine 
Lysis method:Pre-filled 0.5 mm glass bead tubes on a bead mill homogenizer or vortex mixer 
Equipment required:Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol