Description
PRODUCT DESCRIPTION
Bead Mill Soil DNA Purification Midi Kit – 50 Prep
The Omni Soil DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from soil and sediments. After sample lysis using glass beads, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Soil DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.
Product Features:
Complete DNA purification kit including spin-columns and nontoxic reagents
Effective removal of PCR inhibitors including humic compounds
Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
Isolation of pure DNA from up to 1 g soil, clay, sediment and compost
Recover up to 25 ug DNA per filter
Illustrated Protocol
Ordering Information: Unit includes 50 spin-columns, bulk 0.5 mm glass, reagents suitable for 50 purifications and instruction guide. Note: 15 mL conical tubes not included
Specifications
DNA types:Microbial genomic DNA
DNA purity:High quality genomic DNA with A260/A280 > 1.8
Inhibitor removal:DNA is separated from humic compounds and other PCR inhibitors prior to purification
DNA yield:Up to 25 ug per filter
Capacity:Capable of microbial DNA extraction from up to 1 g of soil and sediment
Lysis method:0.5 mm glass beads on a bead mill homogenizer or vortex mixer
Equipment required:Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol
Accessories
15 mL Tubes with Screw Caps
15 mL Tubes with Screw Caps
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