ALT/GPT Chemistry Reagents

ALT/GPT Chemistry Reagents Brand: Cromatest PRINCIPLE? Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by? lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the activity of ALT present in the sample. Product Overview IFCC standards:?This test has been formulated according the standardized method described by IFCC. Clin Chem Lab Med 2002; 40(7) : 718-7242 UV enzymatic method Kinetic ALT/GPT 3 x 100ml Storage: at 2-8?C REAGENT COMPOSITION R1 ALT substrate: TRIS buffer 150 mmol/L pH 7.3, L-alanine 750 mmol/L, lactate dehydrogenase? 1350 U/L. R2 ALT coenzyme. NADH 1.3 mmol/L, 2-oxoglutarate 75 mmol/L. Biocides. REAGENT PREPARATION Working reagent. Mix 4 mL of R1 + 1 mL of R2. Stable for 4 weeks at 2-8?C. Protect from light. SAMPLES Serum, EDTA or heparinized plasma free of hemolysis. ALT is stable in serum or plasma 24 hours at room temperature and for 1 week at 2-8?C. MATERIALS REQUIRED Photometer or spectrophotometer with a thermostatic cell compartment set at 30/37?C, capable of reading at 340 nm. Stopwatch, strip-chart recorder or printer. Cuvettes with 1-cm pathlength. Pipettes to measure reagent and samples. ALT/GPT Chemistry Reagents per box