Albumin Clinical Chemistry Reagents

Albumin Clinical Chemistry Reagents Colorimetric method ENDPOINT PRINCIPLE The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH with the resulting shift in the absorption wavelength of the complex. The? intensity of the color formed is proportional to the concentration of albumin in the sample. REAGENT COMPOSITION R1 Bromocresol reagen: . Succinate buffer 75 mmol/L pH 4.3, BCG 0.12 mmol/L, tensioactive 2 g/L (w/v). 4 x 100ml CAL Albumin standard:?Bovine serum albumin 5 g/dL (50 g/L) Concentration value is traceable to Standard Reference Material 927c. 1 x 3ml STORAGE AND STABILITY Store at 2-8?C REAGENT PREPARATION The Reagent and Standard are ready-to-use. SAMPLES Serum, EDTA plasma unhemolyzed. Albumin in serum and plasma is stable for 2 weeks at 2-8?C, and for up to 4 months at ?20?C MATERIALS REQUIRED Photometer or colorimeter capable of measuring absorbance at 630 ? 20 nm. Pipettes to measure reagent and samples. Timer. This is not necessary if the assay is performed in an automated instrument. Albumin Clinical Chemistry Reagents