Using CRISPR to Treat Cystic Fibrosis

Using CRISPR to Treat Cystic Fibrosis

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

Group Size: For 8 Lab Groups
Storage: Room Temperature Stable. Storage of Ready-to-Load Quicks trip™ samples in the refrigerator is recommended
Time Required: Complete in 45 minutes

The kit includes:

Instructions,
Ready-to-Load Quick Strip™ DNA Samples
Ultra Spec-Agarose™
Electrophoresis Buffer (50X)
10X Gel Loading Solution,
Flash Blue™ DNA Stain

Additional required equipment & materials (not supplied with the kit):

DNA Electrophoresis tank and accessories including casting trays and special power supply
Micropipettes: 5-50 µl (Optional)
Balance
White Light Box
Microwave or Hot Plate

*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*Reasons to Love
An excellent activity to demonstrate genetics and Cystic Fibrosis Diagnostics and Treatment
Learning Outcomes
A-Level Genetics: Principles & Applications of Electrophoresis
Top Tips
When visualising completed gels using a white light box, cover the light box with clingfilm to prevent any damage.