Composition:
40% glycerol, 0.25% Bromophenol Blue, 6mM Tris-HCl Final ConcentrationThere are no reviews for this product.Write a review
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Material: Polypropylene.
Sterility: Non-sterileThere are no reviews for this product.Write a review
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Product Details? DNA Molecular Weight Marker XIV (100 bp ladder)PROPERTIESformsolutionQuality Level100packagingpkg of 50??g (in 200 ?l)manufacturer/tradenameRocheshipped indry icestorage temp.?20?C?DESCRIPTIONGeneral descriptionFragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range:?100 to 1500 bp and an additional band of 2642 bpApplicationDNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5?-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or?32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.DNA Molecular Weight Marker XIV has been used in gel electrophoresis.SequenceThe mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400,?500, 600, 700, 800, 900,?1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.Unit Definition50 ?g = 1A260?unitPhysical formReady-to-use solution, 250 ?g/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).Other NotesFor life science research only. Not for use in diagnostic procedures.There are no reviews for this product.Write a review
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DNA sequencing is a laboratory technique used to determine the order of nucleotides (A, T, C, G) in a DNA molecule. It is a crucial tool for many areas of research, including genetics, genomics, and molecular biology.
There are different methods for DNA sequencing, but the most commonly used method is called Sanger sequencing, which was developed by Frederick Sanger in 1977. Here are the basic steps involved in Sanger sequencing:
DNA Fragmentation: The DNA molecule is first fragmented into smaller pieces of a few hundred bases in length.
Amplification: Each fragment is then amplified using a technique called PCR (Polymerase Chain Reaction) to produce multiple copies of the fragment.
Labeling: During amplification, a small amount of modified DNA bases (ddNTPs) are added to the reaction. These modified bases terminate DNA strand elongation when incorporated into the growing DNA strand.
Separation: The labeled fragments are then separated based on their size using gel electrophoresis. The fragments are loaded onto a gel matrix, and an electric current is applied to separate the fragments by size. The smallest fragments move the farthest, and the largest fragments remain closest to the origin.
Detection: After separation, the labeled fragments can be detected using fluorescent tags or radioactivity.
Analysis: Finally, the sequence of the DNA molecule can be determined by analyzing the labeled fragments based on their size and position on the gel.
Sanger sequencing is a reliable and accurate method for DNA sequencing, but it is also time-consuming and labor-intensive, and can only sequence a limited length of DNA at a time. In recent years, newer sequencing technologies, such as Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS), have emerged, which offer faster and more comprehensive sequencing of DNA.
DNA Structure Model - 5 Parts Mounted on Base
Set of 5 different models detailing DNA structures are mounted on a common wooden base for classroom observation
Large model with DNA structures magnified 1000's of times provides comprehensive detail of molecule structure, mechanisms of replication, and nucleotide base pairing
Expertly designed by biologists and educators for the highest degree of detail and accuracy
Custom molded from PVC plastic for durability and hand-painted by skilled artists to deliver the highest level of detail
Structures are clearly labeled for easy identification
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Description
Savant SpeedVac DNA 130 Integrated Vacuum Concentrator System
Thermo Scientific Savant SpeedVac DNA130 is a compact, integrated vacuum concentrator designed for fast, efficient concentration of DNA and RNA applications. SpeedVac™ DNA130 vacuum concentrator will support sample preparations with ease and efficiency.
Compact, easy to use design: smallest integrated benchtop unit saves lab bench space.
Corrosion-resistant, PTFE coated chamber offers years of service
Built-in, oil free vacuum pump offers maintenance-free operation
Includes a rotor that holds 36 x 1.5-2.0 ml tubes (RD36)
Operating Features:
Consistent performance: designed to ensure consistent drying times and reproducible results
User-friendly interface
Three user defined programs that can be modified and saved
Temperature set point: ambient, 35°C to 65°C in 5°C increments
Heat Time setting: from 1 m to 9h 59 m or continuous
Run Time setting: from 1m to 9h 59m
Audible alarm to alert user when run is complete
Sample Protection
Dry at ambient temperatures or using low heat to avoid sample denaturing
Oil-free diaphragm pump provides gentle transition from atmosphere to ultimate vacuum setting with no solvent bumping
Safety Features
PTFE-coated chamber resists chemicals and cleans easily
Latched lid and lid lock indicator ensures user safety during operation
Lid stay mechanism to secure lid while it‘s open, allowing user to have both hands free for operation
Unit and pump are virtually maintenance-free
External post trap to neutralize ammonium hydroxide vapors
Optional chemical traps and optional cartridges to trap radioactive chemicals to minimize concerns in handling hazardous fumes and chemicals.
Solvents and Solvent Combinations:
Water
PCR buffers (aqueous)
Ethanol and water
Ammonium Hydroxide (will require an ammonia post-trap ANT-100 and neutralizing solution accessory ANS121)
Product Overview?DNAPac? PA200 Oligonucleotide HPLC Columns
Catalog number: 063000
Related applications: Industrial Chromatography
Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific? DNAPac? PA200 Oligonucleotide HPLC Columns.Product More Specificationsspecification_codeColumn FormatAnalytical ColumnDiameter (Metric)4 mmFlow Rate1.2 mL/minFor Use WithUltiMate 3000 Titanium SystemLength (Metric)250 mmMax. Pressure4000 psi (275 bar)Mobile Phase CompatibilitypH 2 to 12.5, 100% compatible with common organic solvents, Ionic form eluents: chloride, perchlorateParticle Size8 ?mpH2.5 to 12.5Pore SizeNonporousTemperature85?CStationary PhaseQuaternary AmmoniumProduct LineDNAPacPacking MaterialSpherical, Nonporous PolymerColumn TypeIon ExchangePhaseIon ExchangeTypeAnalytical ColumnTechniqueNucleic Acid/Anion ExchangeUnit SizeEachMade InUSAWrite a review
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Applications:
? Removes DNA from protein preparations and RNA samples
? Mediates nick translation
? Generates random fragments for dideoxy sequencing
? Degrade template DNA following in vitro transcription
? Mediate DNase I foot-printingWrite a review
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Product Overview82359
DNase Test Agar BaseConcentration 42.00 gm/lit.
Part D
Storage : 8 to 25?C (Cool & dry area)
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review
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BRODIL SYP 100ML ₦2,059.75
ALLRITE TAB 10`S ₦1,866.00
ATORLIP ASP 20 CAP 10`S ₦830.00
CARIPAYA TAB 10`S ₦9,673.25
CELLCEPT 1GM SUSP ₦210,602.00
GOOD PHARMA S-POWER CREAM (4g) ₦221.00
Natures Aid Cod Liver Oil Omega 3 - 500ml ₦39,000.00
Perfectil Cap ₦1,235.00
Quikhaler Dpi Device 1?s ₦40,800.00
ETOFINE MR CAPSULE ₦5,649.25
Stardea Ansiplus Drops Food Supplement 30ml ₦32,870.00
OZEPRO 1% TUBE OF 5GM CREAM ₦2,554.50
Lusso Tan Tan-Be-Gone Shower Bar ₦30,078.82
CEFF FORTE PREMIX SYP ₦3,807.00
Sterilflor-B? Sterilfarma 12 Sachets ₦30,628.00
Barole 20 caps ₦3,445.00
Composition: 40% glycerol, 0.25% Bromophenol Blue, 6mM Tris-HCl Final ConcentrationThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue![DNA LoBind Microcentrifuge Tubes [PK/250 (0.5ml)]](https://allschoolabs.com/wp-content/uploads/2024/07/2026911000016141019-550x550h-300x300.jpg)
Material: Polypropylene. Sterility: Non-sterileThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product Details? DNA Molecular Weight Marker XIV (100 bp ladder)PROPERTIESformsolutionQuality Level100packagingpkg of 50??g (in 200 ?l)manufacturer/tradenameRocheshipped indry icestorage temp.?20?C?DESCRIPTIONGeneral descriptionFragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases. Size Range:?100 to 1500 bp and an additional band of 2642 bpApplicationDNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination. Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5?-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or?32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.DNA Molecular Weight Marker XIV has been used in gel electrophoresis.SequenceThe mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400,?500, 600, 700, 800, 900,?1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.Unit Definition50 ?g = 1A260?unitPhysical formReady-to-use solution, 250 ?g/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).Other NotesFor life science research only. Not for use in diagnostic procedures.There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
DNA sequencing is a laboratory technique used to determine the order of nucleotides (A, T, C, G) in a DNA molecule. It is a crucial tool for many areas of research, including genetics, genomics, and molecular biology. There are different methods for DNA sequencing, but the most commonly used method is called Sanger sequencing, which was developed by Frederick Sanger in 1977. Here are the basic steps involved in Sanger sequencing:
DNA Structure Model - 5 Parts Mounted on Base Set of 5 different models detailing DNA structures are mounted on a common wooden base for classroom observation Large model with DNA structures magnified 1000's of times provides comprehensive detail of molecule structure, mechanisms of replication, and nucleotide base pairing Expertly designed by biologists and educators for the highest degree of detail and accuracy Custom molded from PVC plastic for durability and hand-painted by skilled artists to deliver the highest level of detail Structures are clearly labeled for easy identification
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Description Savant SpeedVac DNA 130 Integrated Vacuum Concentrator System Thermo Scientific Savant SpeedVac DNA130 is a compact, integrated vacuum concentrator designed for fast, efficient concentration of DNA and RNA applications. SpeedVac™ DNA130 vacuum concentrator will support sample preparations with ease and efficiency. Compact, easy to use design: smallest integrated benchtop unit saves lab bench space. Corrosion-resistant, PTFE coated chamber offers years of service Built-in, oil free vacuum pump offers maintenance-free operation Includes a rotor that holds 36 x 1.5-2.0 ml tubes (RD36) Operating Features: Consistent performance: designed to ensure consistent drying times and reproducible results User-friendly interface Three user defined programs that can be modified and saved Temperature set point: ambient, 35°C to 65°C in 5°C increments Heat Time setting: from 1 m to 9h 59 m or continuous Run Time setting: from 1m to 9h 59m Audible alarm to alert user when run is complete Sample Protection Dry at ambient temperatures or using low heat to avoid sample denaturing Oil-free diaphragm pump provides gentle transition from atmosphere to ultimate vacuum setting with no solvent bumping Safety Features PTFE-coated chamber resists chemicals and cleans easily Latched lid and lid lock indicator ensures user safety during operation Lid stay mechanism to secure lid while it‘s open, allowing user to have both hands free for operation Unit and pump are virtually maintenance-free External post trap to neutralize ammonium hydroxide vapors Optional chemical traps and optional cartridges to trap radioactive chemicals to minimize concerns in handling hazardous fumes and chemicals. Solvents and Solvent Combinations: Water PCR buffers (aqueous) Ethanol and water Ammonium Hydroxide (will require an ammonia post-trap ANT-100 and neutralizing solution accessory ANS121)
Product Overview?DNAPac? PA200 Oligonucleotide HPLC Columns Catalog number: 063000 Related applications: Industrial Chromatography Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific? DNAPac? PA200 Oligonucleotide HPLC Columns.Product More Specificationsspecification_codeColumn FormatAnalytical ColumnDiameter (Metric)4 mmFlow Rate1.2 mL/minFor Use WithUltiMate 3000 Titanium SystemLength (Metric)250 mmMax. Pressure4000 psi (275 bar)Mobile Phase CompatibilitypH 2 to 12.5, 100% compatible with common organic solvents, Ionic form eluents: chloride, perchlorateParticle Size8 ?mpH2.5 to 12.5Pore SizeNonporousTemperature85?CStationary PhaseQuaternary AmmoniumProduct LineDNAPacPacking MaterialSpherical, Nonporous PolymerColumn TypeIon ExchangePhaseIon ExchangeTypeAnalytical ColumnTechniqueNucleic Acid/Anion ExchangeUnit SizeEachMade InUSAWrite a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
There are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue
Product Overview82359 DNase Test Agar BaseConcentration 42.00 gm/lit. Part D Storage : 8 to 25?C (Cool & dry area) HSN Code : 38210000 IMDG Identification :Not Regulated for Transport (Non-Haz)Package Size: 500 GProduct More SpecificationsMade InIndiaThere are no reviews for this product.Write a review Your Name Your ReviewNote: HTML is not translated! Rating Bad Good Captcha Please complete the captcha validation below Continue