Description

Xylanase Assay Kit (XylX6 Method)

The XylX6 assay reagent for the measurement of?endo-xylanase (endo-1,4-?-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-?-D-45-glucosyl-xylopentaoside and 2) ?-xylosidase. The ketone blocking group prevents any hydrolytic action by the ?-xylosidase or other?exo-acting glycosidases on the XylX6 substrate. Incubation with an?endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary ?-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the?endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).